pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP
(Plasmid #111585)

• Purpose: An AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.
• Depositing Lab: Bong-Kiun Kaang
• Publication: Choi et al Science. 2018 Apr 27;360(6387):430-435. doi: 10.1126/science.aas9204.

Backbone

• Vector backbone: pAAV
  (Search Vector Database)
• Backbone size w/o insert (bp): 5293
• Total vector size (bp): 7399
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: myriRFP670V5-P2A-post-eGRASP
• Insert Size (bp): 2106
• Promoter: Ef1a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Resource Information

• Supplemental Documents:
  • pAAV-EWB-DIO-myriRFP670V5-P2A-kSPSH3S11Nlg(d4).gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry