PurposeAn AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||111589||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5293
- Total vector size (bp) 6643
Growth in Bacteria
Growth Strain(s)NEB Stable
Gene/Insert namecyan pre-eGRASP(p32)
Insert Size (bp)1350
- Promoter Ef1a
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer None
- 3′ sequencing primer None (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-EWB-DIO-cyan pre-eGRASP(p32) was a gift from Bong-Kiun Kaang (Addgene plasmid # 111589 ; http://n2t.net/addgene:111589 ; RRID:Addgene_111589)
For your References section:Interregional synaptic maps among engram cells underlie memory formation. Choi JH, Sim SE, Kim JI, Choi DI, Oh J, Ye S, Lee J, Kim T, Ko HG, Lim CS, Kaang BK. Science. 2018 Apr 27;360(6387):430-435. doi: 10.1126/science.aas9204. 10.1126/science.aas9204 PubMed 29700265