165,637 results

1. pRK24

   Bacterial Strain

   #51950

   Purpose

   broad-host range natural conjugative plasmid

   Depositor

   Farren Isaacs

   Article

   Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

   Bacterial Resistance

   Tetracycline

   Available since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

2. FERM domain of Ezrin

   Plasmid

   #62380

   Purpose

   Expresses the FERM domain of Ezrin (N terminal portion of the protein)

   Depositor

   Guillaume Charras

   Article

   Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

   Insert

   Ezrin aa1-309 (EZR Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains FERM domain of Ezrin (N terminal portion…

   Promoter

   CMV

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-EGFP

   Plasmid

   #21181

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

   Insert

   enhanced green flourescent protein
   

   Use

   Drosophila expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDC191

   Plasmid

   #115792

   Purpose

   Expresses Rat synaptobrevin 2

   Depositor

   Axel Brunger

   Article

   Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

   Insert

   Synaptobrevin2 (Vamp2 Rat)
   

   Use

   Tags

   hexa histidine

   Expression

   Bacterial

   Mutation

   S28C and C103S (please see comments below)

   Promoter

   T7

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDC158

   Plasmid

   #115793

   Purpose

   Expresses Rat SNAP25

   Depositor

   Axel Brunger

   Article

   Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

   Insert

   SNAP25 (Snap25 Rat)
   

   Use

   Tags

   hexa histidine

   Expression

   Bacterial

   Mutation

   c84s, c85s, c90s, c92s

   Promoter

   T7

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRVL-3

   Plasmid

   #104581

   Purpose

   Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions

   Depositor

   Daniel Christ

   Article

   Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

   Insert

   Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L234A/L235E/G237A and E318A/K320A/K322A

   Promoter

   CMV

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)Flag-His-ATM wt

   Plasmid

   #31985

   Purpose

   Mammalian expression of human ATM with Flag and His tags

   Depositor

   Michael Kastan

   Article

   Canman et al Science. 1998 Sep 11;281(5383):1677-9.

   Insert

   ATM wild-type (ATM Human)
   

   Use

   Tags

   Flag and HIS

   Expression

   Mammalian

   Mutation

   3' UTR of 400 nucl.

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-mCherry-Mango II x 24

   Plasmid

   #127587

   Purpose

   Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

   Depositor

   David Rueda

   Article

   Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

   Insert

   mCherry-Mango II x24
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE Tet ON

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCherry.90 alpha

   Plasmid

   #108222

   Purpose

   Mammalian expression vector for mCherry fusion to human Hsp90 alpha

   Depositor

   Didier Picard

   Article

   Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

   Insert

   mCherry-Hsp90a (HSP90AA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MigR1-AE9a

    Plasmid

    #12433

    Depositor

    Dong-Er Zhang

    Article

    Yan et al Nat Med. 2006 Aug . 12(8):945-9.

    Insert

    AML1-ETO9a fusion (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AML-ETO protein with ETO exon 9a.

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHiUGE GS-gRNA (hSyn-Cre) mTubb3

    Plasmid

    #200369

    Purpose

    Positive control plasmid expressing mouse Tubb3 GS-gRNA (with hSyn-Cre)

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    Mouse Tubb3 gRNA and Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. ePB-PURO-TT-PA-CRE

    Plasmid

    #179584

    Purpose

    ePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    PA-MAG-CRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHiUGE smFP-V5 donor ORF2

    Plasmid

    #200382

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 401 pSG5L

    Plasmid

    #10737

    Depositor

    William Sellers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pTalpha1-Dre

    Plasmid

    #133925

    Purpose

    Neuron-specific expression of Dre

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    HA-Dre
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-6011nes

    Plasmid

    #108652

    Purpose

    An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-ERK-EV
    

    Use

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLIK-PUM1-rABE-Flag

    Plasmid

    #191385

    Purpose

    Doxycycline inducible expression of PUM1-rABE

    Depositor

    Stephen Floor

    Article

    Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

    Insert

    PUM1-rABE (PUM1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    tet-inducible promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G

    Plasmid

    #194458

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-CD19 CAR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-FOXA2

    Plasmid

    #153110

    Purpose

    Expression of Flag tagged human FOXA2 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXA2 (FOXA2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGCS vector kit

    Plasmid Kit

    #1000000107

    Purpose

    A series of pCS2+ based Gateway vectors with N- or C-terminal tags (eg Myc, HA, Flag, GST and eGFP), which allow the generation of tagged proteins in a variety of vertebrate organisms.

    Depositor

    Hai-Ning Du

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only