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pET-21a_3xNLS_SpCas9_protein_expression Citations (7)

Originally described in: Highly efficient therapeutic gene editing of human hematopoietic stem cells.
Wu Y, Zeng J, Roscoe BP, Liu P, Yao Q, Lazzarotto CR, Clement K, Cole MA, Luk K, Baricordi C, Shen AH, Ren C, Esrick EB, Manis JP, Dorfman DM, Williams DA, Biffi A, Brugnara C, Biasco L, Brendel C, Pinello L, Tsai SQ, Wolfe SA, Bauer DE Nat Med. 2019 May;25(5):776-783. doi: 10.1038/s41591-019-0401-y. Epub 2019 Mar 25.
PubMed Journal

Articles Citing pET-21a_3xNLS_SpCas9_protein_expression

Editing aberrant splice sites efficiently restores beta-globin expression in beta-thalassemia. Xu S, Luk K, Yao Q, Shen AH, Zeng J, Wu Y, Luo HY, Brendel C, Pinello L, Chui DHK, Wolfe SA, Bauer DE. Blood. 2019 Jan 31. pii: blood-2019-01-895094. doi: 10.1182/blood-2019-01-895094. PubMed

Associated Plasmids

Precise therapeutic gene correction by a simple nuclease-induced double-stranded break. Iyer S, Suresh S, Guo D, Daman K, Chen JCJ, Liu P, Zieger M, Luk K, Roscoe BP, Mueller C, King OD, Emerson CP Jr, Wolfe SA. Nature. 2019 Apr;568(7753):561-565. doi: 10.1038/s41586-019-1076-8. Epub 2019 Apr 3. PubMed
Genome-wide detection of CRISPR editing in vivo using GUIDE-tag. Liang SQ, Liu P, Smith JL, Mintzer E, Maitland S, Dong X, Yang Q, Lee J, Haynes CM, Zhu LJ, Watts JK, Sontheimer EJ, Wolfe SA, Xue W. Nat Commun. 2022 Jan 21;13(1):437. doi: 10.1038/s41467-022-28135-9. PubMed

Associated Plasmids

Efficient Genome Editing Achieved via Plug-and-Play Adenovirus Piggyback Transport of Cas9/gRNA Complex on Viral Capsid Surface. Lu ZH, Li J, Dmitriev IP, Kashentseva EA, Curiel DT. ACS Nano. 2022 Jul 26;16(7):10443-10455. doi: 10.1021/acsnano.2c00909. Epub 2022 Jun 24. PubMed
Optimized Guide RNA Selection Improves Streptococcus pyogenes Cas9 Gene Editing of Human Hematopoietic Stem and Progenitor Cells. Verhagen HJMP, Kuijk C, Rutgers L, Kokke AM, van der Meulen SA, van Mierlo G, Voermans C, van den Akker E. CRISPR J. 2022 Oct;5(5):702-716. doi: 10.1089/crispr.2021.0112. Epub 2022 Sep 28. PubMed
Highly Efficient One-Step Tagging of Endogenous Genes in Primary Cells Using CRISPR-Cas Ribonucleoproteins. Yao Y, Cao J, Wang W, Liu B, Pei X, Zhang L, Rao S. CRISPR J. 2022 Dec;5(6):843-853. doi: 10.1089/crispr.2022.0046. Epub 2022 Nov 24. PubMed
Human genetic diversity alters off-target outcomes of therapeutic gene editing. Cancellieri S, Zeng J, Lin LY, Tognon M, Nguyen MA, Lin J, Bombieri N, Maitland SA, Ciuculescu MF, Katta V, Tsai SQ, Armant M, Wolfe SA, Giugno R, Bauer DE, Pinello L. Nat Genet. 2023 Jan;55(1):34-43. doi: 10.1038/s41588-022-01257-y. Epub 2022 Dec 15. PubMed

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