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Directed Evolution of Split APEX2 Peroxidase.
Han Y, Branon TC, Martell JD, Boassa D, Shechner D, Ellisman MH, Ting A
ACS Chem Biol. 2019 Mar 8. doi: 10.1021/acschembio.8b00919. PubMed Article

Plasmids from Article

ID Plasmid Purpose
120912FKBP-V5-AP-nes_pLX304Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol
120913HA-Halotag-FRB-EX-nes_pLX304Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol
120914Tom20-V5-FKBP-AP_pLX304Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membrane
120915EX-HA-FRB-Cb5_pLX304Lentiviral vector expressing EX-HA-FRB-Cb5 on mammalian ER membrane
120917His6x-EX-HA-FRB-MBD_pYFJ16Expresses His6-EX-HA-FRB-MBD in bacteria for protein purification
120918NLS-MCP-AP_pLX304Lentiviral vector expressing NLS-MCP-AP in mammalian nucleus

Antibodies from Article