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PurposeReporter gene with mouse c-jun promoter.
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Depositing Lab
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Depositing OrganizationColumbia University
Located in United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11979 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 11979-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 11979-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL3
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namec-jun promoter
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Alt namec-jun
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SpeciesM. musculus (mouse)
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Insert Size (bp)375
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Entrez GeneJun (a.k.a. AP-1, Junc, c-jun)
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Tag
/ Fusion Protein
- Luc (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer NA
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmids pJC6GL3, pJSXGL3, pJTXGL3, and pJSTXGL3 contain positions -225 to +150 of the murine c-jun promoter.
DNA (Catalog # 11979-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 11979-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJC6-GL3 was a gift from Ron Prywes (Addgene plasmid # 11979 ; http://n2t.net/addgene:11979 ; RRID:Addgene_11979) -
For your References section:
Epidermal growth factor induction of the c-jun promoter by a Rac pathway. Clarke N, Arenzana N, Hai T, Minden A, Prywes R. Mol Cell Biol. 1998 Feb . 18(2):1065-73. PubMed 9448004
Map uploaded by the depositor.
