PurposeReporter gene with mouse c-fos promoter. Induced by serum and many growth factors in quiescent cells.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11983||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepGL3-promoter modified
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namec-fos promoter
SpeciesM. musculus (mouse)
Insert Size (bp)465
Entrez GeneFos (a.k.a. D12Rfj1, c-fos, cFos)
/ Fusion Protein
- Luc (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site See author map (destroyed during cloning)
- 3′ cloning site See author map (destroyed during cloning)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
See author's map for cloning details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFOS WT-GL3 was a gift from Ron Prywes (Addgene plasmid # 11983 ; http://n2t.net/addgene:11983 ; RRID:Addgene_11983)
For your References section:Megakaryoblastic leukemia 1, a potent transcriptional coactivator for serum response factor (SRF), is required for serum induction of SRF target genes. Cen B, Selvaraj A, Burgess RC, Hitzler JK, Ma Z, Morris SW, Prywes R. Mol Cell Biol. 2003 Sep . 23(18):6597-608. 10.1128/MCB.23.18.6597-6608.2003 PubMed 12944485