pCGN-SRF
(Plasmid #11977)

• Purpose: For mammalian expression of human SRF with an HA tag
• Depositing Lab: Ron Prywes
• Publication: Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

Backbone

• Vector backbone: pCGN
• Backbone size w/o insert (bp): 7500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SRF
• Species: H. sapiens (human)
• Entrez Gene: SRF (a.k.a. MCM1)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: Bglob-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map and paper for cloning details. pCGN: expression is driven by CMV promoter