164,349 results

1. pLV_3XHA-ATP6V1B2-mNeonGreen

   Plasmid

   #228865

   Purpose

   Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

   Depositor

   Wilhelm Palm

   Article

   Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

   Insert

   ATP6V1B2 (ATP6V1B2 Human)
   

   Use

   Lentiviral

   Tags

   3xHA and mNeonGreen

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a-alpha-synuclein

   Plasmid

   #51486

   Purpose

   Inducible expression of human alpha-synuclein in E.coli

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 31, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLenti-CMV-Puro-PNPT1 S484A

   Plasmid

   #223316

   Purpose

   Lentiviral vector expressing human PNPT1 S484A variant

   Depositor

   Judy Lieberman

   Article

   Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

   Insert

   Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed serine 484 to alanine

   Promoter

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmScarlet3-Giantin_C1

   Plasmid

   #189773

   Purpose

   Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-Giantin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (AAV9)

   Viral Prep

   #162382-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (#162382). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of the ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   CAG

   Tags

   None

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

6. AAV-hSyn-Soma-jGCaMP8m

   Plasmid

   #169257

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   Soma-jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNIC28-Bsa4

   Plasmid

   #26103

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-Cas9-gRNA-TagBFP2

   Plasmid

   #124774

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (AAV8)

   Viral Prep

   #84446-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (#84446). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] plasmid DNA. CAG-driven, Cre-dependent expression of Jaws-KGC-tdTomato-ER2 for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Promoter

   CAG

   Tags

   None

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-hSyn-ConVERGD-(HA)hM4Di-W3SL

    Plasmid

    #218759

    Purpose

    Provides AND intersectional (Cre+Flp) expression of HA-hM4Di

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    hM4Di
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJSK659

    Plasmid

    #205128

    Purpose

    Expression of GFP-SnxA in mammalian cells

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    SnxA
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDisplay-NE2h-IRES-mCherry-CAAX

    Plasmid

    #208691

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cells

    Depositor

    Yulong Li

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 Ash2L full

    Plasmid

    #15548

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    Ash2L (ASH2L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R622H (Please see depositor comments below)

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ii-Str_ssSBP-EGFP

    Plasmid

    #65277

    Purpose

    to synchronize transport of secretory soluble GFP from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    EGFP
    

    Use

    Tags

    IL-2 signal sequence and Streptavidin Binding Pr…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Man2a1-GFP

    Plasmid

    #163649

    Purpose

    Expresses GFP tagged Man2a1 in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mannosidase alpha class 2A member 1 (MAN2A1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Silent mutation was made to introduce a XbaI rest…

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS U3-hTR-500

    Plasmid

    #28170

    Depositor

    Kathleen Collins

    Article

    Fu et al Mol Cell. 2003 May . 11(5):1361-72.

    Insert

    U3-hTR-500 (TERC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. μ2-HA-WT

    Plasmid

    #32752

    Depositor

    Alexander Sorkin

    Article

    Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

    Insert

    μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

    Plasmid

    #177920

    Purpose

    Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-F30-Broccoli-Squash-SAM-sensor 5-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-KG-D4H*-mCherry

    Plasmid

    #134604

    Purpose

    fluorescent cholesterol-binding probe

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    D4H*
    

    Use

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    Y415A and A463W

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. TPC2-mCherry

    Plasmid

    #135183

    Purpose

    Expresses TPC2 with C-terminus mCherry tag in mammalian cells

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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