161,280 results

1. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV5)

   Viral Prep

   #162381-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   CAG

   Tags

   None

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

2. pHIV-iRFP720-E2A-Luc

   Plasmid

   #104587

   Purpose

   Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

   Depositor

   Antonius Plagge

   Article

   Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

   Insert

   iRFP720-E2A-Luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   bi-cistronic

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. TPC1-G-GECO1.2

   Plasmid

   #207143

   Purpose

   Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   TPCN1 (TPCN1 Human)
   

   Use

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCTcon2

   Plasmid

   #41843

   Depositor

   Dane Wittrup

   Article

   Chao et al Nat Protoc. 2006;1(2):755-68.

   Insert

   CD20
   

   Use

   Tags

   HA and c-myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9_GFP

   Plasmid

   #44719

   Purpose

   Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP shRNA tet pLKO puro

   Plasmid

   #163017

   Purpose

   Control tet-inducible shRNA targeting eGFP

   Depositor

   Mark Rubin

   Article

   Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNDL-54

   Plasmid

   #161715

   Purpose

   In vitro transcription construct for production of membrane acvr1b-Cry2 mRNA

   Depositor

   Adam Cohen

   Article

   McNamara et al bioRxiv [Preprint]. 2024 Apr 12:2024.04.

   Insert

   Myr-acvr1b-Cry2 (acvr1ba Zebrafish)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNDL-60

   Plasmid

   #161720

   Purpose

   In vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNA

   Depositor

   Adam Cohen

   Article

   McNamara et al bioRxiv [Preprint]. 2024 Apr 12:2024.04.

   Insert

   cytosolic acvr2b-CIB1N (acvr2b Zebrafish)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. 4xmts-G-Ca-FLITS

   Plasmid

   #191462

   Purpose

   Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   G-Ca-FLITS
   

   Use

   Tags

   4xmts

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEED

    Plasmid

    #219658

    Purpose

    Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Empty SEED Cassette (3xP3-dsRed)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-CMV-GCaMP6s-CAAX

    Plasmid

    #52228

    Purpose

    Plasma membrane targeted GCaMP6s, calcium sensor

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    GCaMP6s-CAAX
    

    Use

    Tags

    6xHis and CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-C-STAT1-mGFP-P2A-Puro

    Plasmid

    #199345

    Purpose

    express murine STAT1 protein fused with mGFP

    Depositor

    Massimo Mallardo

    Article

    Caiazza et al Int J Mol Sci. 2022 Nov 17;23(22):14232.

    Insert

    signal transducer and activator of transcription 1 (Stat1 Mouse)
    

    Use

    Lentiviral

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV_IRES_GFP_CD16a

    Plasmid

    #196189

    Purpose

    Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

    Depositor

    Ana Ruiz-Saenz

    Article

    Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

    Insert

    CD16a (FCGR3A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 158 to Valine

    Promoter

    LTR

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-PARP1

    Plasmid

    #215136

    Purpose

    Expresses mCherry-PARP1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-Lamin-A

    Plasmid

    #206027

    Purpose

    Expresses GFP-tagged Lamin A in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Lamin-A (LMNA Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. plentiCMV-sp-HPV-TM(ICAM)

    Plasmid

    #196510

    Purpose

    Express HPV antigen epitope with ICAM transmembrane domain

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    a HPV epitope with CD8a stalk linker and ICAM TM domain
    

    Use

    Lentiviral

    Tags

    3xflag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-GRAB_r5-HT1.0

    Plasmid

    #208719

    Purpose

    Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Red fluorescent 5-HT sensor GRAB_r5-HT1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUbC-nls-ha-stdMCP-stdGFP

    Plasmid

    #98916

    Purpose

    synonymized tandem dimer MCP fused to synonymized tandem dimer GFP

    Depositor

    Robert Singer

    Article

    Wu et al Genes Dev. 2015 Apr 15;29(8):876-86. doi

    Insert

    stdMCP-stdGFP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL4.82(hRluc/Puro)

    Plasmid

    #212933

    Purpose

    Renilla luciferase vector for investigating regions controlling transcription

    Depositor

    Pete Stecha

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pYS17

    Plasmid

    #202083

    Purpose

    Parent vector for producing universal minicircle donors for mNeonGreen N-terminal knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    NT PuroR-T2A-mNG Minicircle Parent
    

    Use

    CRISPR

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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