|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17624||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 11500
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesH. sapiens (human)
Insert Size (bp)850
Entrez GeneHES1 (a.k.a. HES-1, HHL, HRY, bHLHb39)
- Cloning method Restriction Enzyme
- 5′ sequencing primer EF-1a Forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
hHES1 driven by the EF-1a promoter and a GFP reporter driven by the ubiquitin C (Ubc) promoter.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EF.hHES1.Ubc.GFP was a gift from Linzhao Cheng (Addgene plasmid # 17624 ; http://n2t.net/addgene:17624 ; RRID:Addgene_17624)
For your References section:HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Yu X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI. Stem Cells. 2006 Apr . 24(4):876-88. 10.1634/stemcells.2005-0598 PubMed 16513761