|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15950||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8263
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsExpand pLOVE/GeneX in Stbl3 cells(sensitive to ccdB)
Copy numberHigh Copy
Gene/Insert nameKruppel-like factor 4
Alt nameKlf4, GKLF, EZF
SpeciesM. musculus (mouse)
Insert Size (bp)2156
MutationThe recombination reaction required to clone "Gene X" into the pLOVE vector backbone removes 1456 bp (the CmR-ccdB region). Therefore, the size of pLOVE-Klf4 is ~8963 bp.
Entrez GeneKlf4 (a.k.a. EZF, Gkl, Gklf, Zi, Zie)
/ Fusion Protein
- V5 (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer V5 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLOVE-Klf4 was a gift from Miguel Ramalho-Santos (Addgene plasmid # 15950 ; http://n2t.net/addgene:15950 ; RRID:Addgene_15950)
For your References section:Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection. Blelloch R, Venere M, Yen J, Ramalho-Santos M. Cell Stem Cell. 2007 Sep 13. ():. 10.1016/j.stem.2007.08.008 PubMed 18371358