Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing.
Anzalone AV, Gao XD, Podracky CJ, Nelson AT, Koblan LW, Raguram A, Levy JM, Mercer JAM, Liu DR
Nat Biotechnol. 2021 Dec 9. pii: 10.1038/s41587-021-01133-w. doi: 10.1038/s41587-021-01133-w. PubMed Article
Anzalone AV, Gao XD, Podracky CJ, Nelson AT, Koblan LW, Raguram A, Levy JM, Mercer JAM, Liu DR
Nat Biotechnol. 2021 Dec 9. pii: 10.1038/s41587-021-01133-w. doi: 10.1038/s41587-021-01133-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 181923 | attB-puro donor.dna | attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion |
| 182138 | attB-puro-GA donor | attB-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion |
| 182139 | attP-puro donor | attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion |
| 182140 | attP-puro-GA donor | attP-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion |
| 182141 | Factor IX donor | Factor IX donor for twinPE and Bxb1 mediated insertion at ALB |
| 182142 | pCMV-Bxb1 | Expression of human codon optimized Bxb1 |
| 182143 | pU6-Sp-gRNA-AAVS1_A3786c_attP | To insert attP attachment site at AAVS1 in human cells via twinPE |
| 182144 | pU6-Sp-gRNA-AAVS1_B3903c_attP | To insert attP attachment site at AAVS1 in human cells via twinPE |
| 182145 | pU6-Sp-gRNA-ALB_DF_A277c_attB | To insert attB attachment site at ALB in human cells via twinPE |
| 182146 | pU6-Sp-gRNA-ALB_DF_B358c_attB | To insert attB attachment site at ALB in human cells via twinPE |
| 182147 | pU6-Sp-gRNA-CCR5_DF_A509b_attB | To insert attB attachment site at CCR5 in human cells via twinPE |
| 182148 | pU6-Sp-gRNA-CCR5_DF_B584b_attB | To insert attB attachment site at CCR5 in human cells via twinPE |
| 182149 | pU6-Sp-gRNA-IDS2_DF_A4b_attP_fwd | To insert attP-fwd attachment site at IDS2 in human cells via twinPE |
| 182150 | pU6-Sp-gRNA-IDS2_DF_B7b_attP_fwd | To install attP-fwd attachment site at IDS2 in human cells via twinPE |
| 182151 | pU6-Sp-gRNA-IDS_DF_C2c_attB_rev | To insert attB-rev attachment site at IDS in human cells via twinPE |
| 182152 | pU6-Sp-gRNA-IDS_DF_D1b_attB_rev | To insert attB-rev attachment site at IDS in human cells via twinPE |