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Optogenetic control of phosphoinositide metabolism.
Idevall-Hagren O, Dickson EJ, Hille B, Toomre DK, De Camilli P
Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 10.1073/pnas.1211305109. Epub 2012 Jul 30.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
66836mCherry-CRY2-5ptaseOCRLMammalian Expression, Bacterial Expression
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66837mCherry-CRY2-5ptaseOCRL(D523G)Mammalian Expression, Bacterial Expression
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66839mCherry-CRY2-iSH2Mammalian Expression, Bacterial Expression
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66841iRFP-PH-PLCdelta1Mammalian Expression, Bacterial Expression
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79561mCherry-CRY2-INPP5Eexpression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif
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79562CRY2-INPP5Eexpression of CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif
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79563mCherry-CRY2-INPP5E(D556A)expression of mCherry-tagged CRY2PHR fused to catalytically dead INPP5E
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79564CRY2-INPP5E(D556A)expression of CRY2PHR fused to catalytically dead INPP5E
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79565GFP-CRY2-OCRLexpression of GFP-tagged CRY2PHR fused to inositol 5-phosphatase domain of OCRL
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79567CRY2-OCRL(D523G)expression of CRY2PHR fused to phosphatase dead mutant of OCRL
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79571CIBN-GFP-TMDexpression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)
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79572Lyn11-CIBN-GFPexpression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP
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79574CIBN-CAAXexpression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting
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