PurposeMammalian Expression, Bacterial Expression
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||66841||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backboneiRFP (31857; Addgene) was amplified with flanking Nhe1/BsrG1 sites and used to replace GFP in pEGFP-C1.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
SpeciesR. norvegicus (rat)
/ Fusion Protein
- iRFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
- 5′ sequencing primer Unknown primer sequence (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:iRFP-PH-PLCdelta1 was a gift from Pietro De Camilli (Addgene plasmid # 66841 ; http://n2t.net/addgene:66841 ; RRID:Addgene_66841)
For your References section:Optogenetic control of phosphoinositide metabolism. Idevall-Hagren O, Dickson EJ, Hille B, Toomre DK, De Camilli P. Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 10.1073/pnas.1211305109. Epub 2012 Jul 30. 10.1073/pnas.1211305109 PubMed 22847441