Purposeexpression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79572||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Alt name11 N-terminal amino acid residues of Lyn kinase
SpeciesH. sapiens (human)
MutationG8R (please see depositor comments below)
- Promoter CMV
/ Fusion Proteins
- CIBN (CRY2-binding domain) (C terminal on backbone)
- EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
CIBN was fused N-terminally to GFP in pEGFP-N1 using Nhe1/Age1. Complementary oligonucleotides encoding Lyn11 with flanking Nhe1-sites subsequently were annealed and ligated N-terminally to CIBN. Lipidation for plasma membrane attachment occurs at amino acids 2 and 3. The G8R mutations stabilise the protein at the plasma membrane.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lyn11-CIBN-GFP was a gift from Pietro De Camilli & Olof Idevall-Hagren (Addgene plasmid # 79572 ; http://n2t.net/addgene:79572 ; RRID:Addgene_79572)
For your References section:Optogenetic control of phosphoinositide metabolism. Idevall-Hagren O, Dickson EJ, Hille B, Toomre DK, De Camilli P. Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 10.1073/pnas.1211305109. Epub 2012 Jul 30. 10.1073/pnas.1211305109 PubMed 22847441