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Engineered Cpf1 variants with altered PAM specificities.
Gao L, Cox DBT, Yan WX, Manteiga JC, Schneider MW, Yamano T, Nishimasu H, Nureki O, Crosetto N, Zhang F
Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
89351pcDNA3.1-hAsCpf1(TYCV) (pY210)Expresses humanized AsCpf1 variant that recognizes TYCV PAMs
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89352pAsCpf1(TYCV)(BB) (pY211)Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide
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89353pcDNA3.1-hAsCpf1(TATV) (pY220)Expresses humanized AsCpf1 variant that recognizes TATV PAMs
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89354pAsCpf1(TATV)(BB) (pY221)Expresses humanized AsCpf1 TATV PAM variant and crRNA guide
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89355pcDNA3.1-hLbCpf1(TYCV) (pY230)Expresses humanized LbCpf1 variant that recognizes TYCV PAMs
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