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pcDNA3.1-hAsCpf1(TYCV) (pY210)
(Plasmid #89351)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89351 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5402
  • Total vector size (bp) 9509
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Acidaminococcus sp. Cpf1 (RR variant)
  • Species
    Synthetic
  • Insert Size (bp)
    4107
  • Mutation
    S542R/K607R
  • Promoter CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    • 3xHA (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

This variant also recognizes CCCC and ACCC PAMs (and, with lower efficiency, VYCV).

For more information on Zhang lab CRISPR plasmids, please refer to www.addgene.org/crispr/zhang/.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-hAsCpf1(TYCV) (pY210) was a gift from Feng Zhang (Addgene plasmid # 89351 ; http://n2t.net/addgene:89351 ; RRID:Addgene_89351)
  • For your References section:

    Engineered Cpf1 variants with altered PAM specificities. Gao L, Cox DBT, Yan WX, Manteiga JC, Schneider MW, Yamano T, Nishimasu H, Nureki O, Crosetto N, Zhang F. Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900. 10.1038/nbt.3900 PubMed 28581492