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pAsCpf1(TYCV)(BB) (pY211)
(Plasmid #89352)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89352 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 89352-D50 50 μg of DNA in Tris buffer $495
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DNA 89352-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pUC ori vector
  • Backbone size w/o insert (bp) 4328
  • Total vector size (bp) 8435
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cpf1, RR variant
  • Alt name
    AsCas12a, RR variant
  • Species
    Synthetic; Acidaminococcus sp.
  • Insert Size (bp)
    4107
  • Mutation
    S542R/K607R
  • Promoter CBh
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS-3xHA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This variant also recognizes CCCC and ACCC PAMs (and, with lower efficiency, VYCV).

Contains a U6-crRNA expression cassette (BbsI restriction sites for guide cloning).

For more information on Zhang lab CRISPR plasmids, please refer to www.addgene.org/crispr/zhang/.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAsCpf1(TYCV)(BB) (pY211) was a gift from Feng Zhang (Addgene plasmid # 89352 ; http://n2t.net/addgene:89352 ; RRID:Addgene_89352)

  • For your References section:

    Engineered Cpf1 variants with altered PAM specificities. Gao L, Cox DBT, Yan WX, Manteiga JC, Schneider MW, Yamano T, Nishimasu H, Nureki O, Crosetto N, Zhang F. Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900. 10.1038/nbt.3900 PubMed 28581492
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