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pAsCpf1(TATV)(BB) (pY221)
(Plasmid #89354)


Item Catalog # Description Quantity Price (USD)
Plasmid 89354 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pUC ori vector
  • Backbone size w/o insert (bp) 4328
  • Total vector size (bp) 8435
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
    Acidaminococcus sp. Cpf1 (RVR variant)
  • Species
  • Insert Size (bp)
  • Mutation
  • Promoter CBh
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    • 3xHA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This variant also recognizes TTTV and RATR PAMs (R = A or G).

Contains a U6-crRNA expression cassette (BbsI restriction sites for guide cloning).

For more information on Zhang lab CRISPR plasmids, please refer to

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAsCpf1(TATV)(BB) (pY221) was a gift from Feng Zhang (Addgene plasmid # 89354 ; ; RRID:Addgene_89354)
  • For your References section:

    Engineered Cpf1 variants with altered PAM specificities. Gao L, Cox DBT, Yan WX, Manteiga JC, Schneider MW, Yamano T, Nishimasu H, Nureki O, Crosetto N, Zhang F. Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900. 10.1038/nbt.3900 PubMed 28581492