PurposeBacterial expression plasmid for protein purification
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||90095||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6643
- Total vector size (bp) 10710
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Specieshumanized Acidaminococcus sp. BV3L6 Cpf1
Insert Size (bp)4056
/ Fusion Proteins
- 6xHis (N terminal on backbone)
- MBP (N terminal on insert)
- TEV site (N terminal on insert)
- Nucleoplasmin NLS (C terminal on insert)
- 3xHA (C terminal on insert)
Note: a Flag-AviTag containing fragment follows the insert (between the XhoI and HindIII sites) but this sequence is not translated.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:6His-MBP-TEV-huAsCpf1 was a gift from Feng Zhang (Addgene plasmid # 90095 ; http://n2t.net/addgene:90095 ; RRID:Addgene_90095)
For your References section:Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F. Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5. 10.1038/nbt.3737 PubMed 27918548