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6His-MBP-TEV-huLbCpf1
(Plasmid #90096)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 90096 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-28
  • Backbone size w/o insert (bp) 3819
  • Total vector size (bp) 10473
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21(DE3)pLysS
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    huLbCpf1
  • Species
    humanized Lachnospiraceae bacterium ND2006 Cpf1
  • Insert Size (bp)
    3819
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • MBP (N terminal on insert)
    • TEV site (N terminal on insert)
    • Nucleoplasmin NLS (C terminal on insert)
    • 3xHA (C terminal on insert)

Resource Information

Depositor Comments

Note: a Flag-AviTag containing fragment follows the insert (between the XhoI and HindIII sites) but this sequence is not translated.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6His-MBP-TEV-huLbCpf1 was a gift from Feng Zhang (Addgene plasmid # 90096 ; http://n2t.net/addgene:90096 ; RRID:Addgene_90096)
  • For your References section:

    Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F. Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5. 10.1038/nbt.3737 PubMed 27918548