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CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, Li Y, Wang R, Yang S
Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
85542pJYS3_ΔcrtYfConstitutive transcription of FnCpf1 and crRNA of crtYf
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85543pJYS2cm_crtYfConstitutive transcription of crRNA of crtYf, Chloramphenicol resistance
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85544pJYS2_crtYfConstitutive transcription of crRNA of crtYf, Spectinomycin resistance
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85545pJYS1PtacConstitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter
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85546pJYS1PeftuConstitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter
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89077pJYS2.1-crtYfConstitutive transcription of crRNA of crtYF. CRISPR of C. glutamicum.
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