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PurposeConstitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85546 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepXMJ19
- Backbone size w/o insert (bp) 5600
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Vector typeBacterial Expression, CRISPR ; Shuttle vector Corynebacterium glutamicum / E. coli
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameCpf1
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SpeciesFrancisella tularensis subsp. novicida
- Promoter etfu
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namerecT
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Speciesunknown
- Promoter unknown
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJYS1Peftu was a gift from Sheng Yang (Addgene plasmid # 85546 ; http://n2t.net/addgene:85546 ; RRID:Addgene_85546) -
For your References section:
CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, Li Y, Wang R, Yang S. Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179. 10.1038/ncomms15179 PubMed 28469274
