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PurposeExpression vector for production of codon optimized Tol2 transposase
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133032 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCS2+
- Total vector size (bp) 6034
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Vector typeIn Vitro Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCodon optimized Tol2 Transposase
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1947
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MutationCodon Optimized
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GenBank IDXP_023805738
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2FA CO Tol2 TPase was a gift from Charles Nathan Hancock (Addgene plasmid # 133032 ; http://n2t.net/addgene:133032 ; RRID:Addgene_133032) -
For your References section:
Codon optimized Tol2 transposase results in increased transient expression of a crystallin-GFP transgene in zebrafish. Mackey AS, Redd PS, DeLaurier A, Hancock CN. MicroPubl Biol. 2020 Jun 11;2020:10.17912/micropub.biology.000268. doi: 10.17912/micropub.biology.000268. 10.17912/micropub.biology.000268 PubMed 32626847
Map uploaded by the depositor.
