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Addgene

pCS2FA CO Tol2 TPase
(Plasmid #133032)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133032 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Total vector size (bp) 6034
  • Vector type
    In Vitro Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Codon optimized Tol2 Transposase
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1947
  • Mutation
    Codon Optimized
  • GenBank ID
    XP_023805738

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2FA CO Tol2 TPase was a gift from Charles Nathan Hancock (Addgene plasmid # 133032 ; http://n2t.net/addgene:133032 ; RRID:Addgene_133032)
  • For your References section:

    Codon optimized Tol2 transposase results in increased transient expression of a crystallin-GFP transgene in zebrafish. Mackey AS, Redd PS, DeLaurier A, Hancock CN. MicroPubl Biol. 2020 Jun 11;2020:10.17912/micropub.biology.000268. doi: 10.17912/micropub.biology.000268. 10.17912/micropub.biology.000268 PubMed 32626847