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Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.

Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 10.1038/s41564-018-0327-z. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
119239pJMP1039expresses Tn7 transposase
119240pJMP1050construction intermediate
119241pJMP1054construction intermediate
119242pJMP1055construction intermediate
119243pJMP1067rfp "test" strain
119244pJMP1069rfp "test" strain
119245pJMP1071rfp "test" strain
119246pJMP1102knockdown phzA1 in P. aeruginosa
119247pJMP1103knockdown phzM in P. aeruginosa
119248pJMP1104knockdown pqsC in P. aeruginosa
119249pJMP1106construction intermediate
119250pJMP1159rfp "test" strain
119251pJMP1161rfp "test" strain
119252pJMP1170rfp "test" strain
119253pJMP1171rfp "test" strain
119254pJMP1183rfp "test" strain
119255pJMP1185rfp "test" strain
119256pJMP1187rfp "test" strain
119257pJMP1189rfp "test" strain
119258pJMP1217rfp "test" strain
119259pJMP1219rfp "test" strain
119260pJMP1221rfp "test" strain
119261pJMP1223rfp "test" strain
119262pJMP1237for cloning new sgRNAs
119263pJMP1239knockdown folA in P. aeruginosa
119264pJMP1263B. subtilis attTn7 site and flanking sequence cloned into pACYC
119265pJMP1273E. coli attTn7 site with B. subtilis flanking sequence cloned into pACYC
119266pJMP1274B. subtilis deltaTn7 site and flanking sequence cloned into pACYC
119267pJMP1290construction intermediate
119268pJMP1333rfp "test" strain
119269pJMP1335rfp "test" strain
119270pJMP1337for cloning new sgRNAs
119271pJMP1339for cloning new sgRNAs
119272pJMP1341control sgRNA in cells lacking rfp
119273pJMP1344knockdown folA in E. aerogenes
119274pJMP1346knockdown folA in K. pneumoniae
119275pJMP1354for cloning new sgRNAs
119276pJMP1356for cloning new sgRNAs
119277pJMP1358for cloning new sgRNAs
119278pJMP1360for cloning new sgRNAs
119279pJMP1363for stabilizing ICE in the presence of rapI expression

Antibodies from Article