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Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 10.1038/s41564-018-0327-z.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
119239pJMP1039expresses Tn7 transposase
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119240pJMP1050construction intermediate
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119241pJMP1054construction intermediate
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119242pJMP1055construction intermediate
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119243pJMP1067rfp "test" strain
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119244pJMP1069rfp "test" strain
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119245pJMP1071rfp "test" strain
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119246pJMP1102knockdown phzA1 in P. aeruginosa
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119247pJMP1103knockdown phzM in P. aeruginosa
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119248pJMP1104knockdown pqsC in P. aeruginosa
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119249pJMP1106construction intermediate
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119250pJMP1159rfp "test" strain
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119251pJMP1161rfp "test" strain
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119252pJMP1170rfp "test" strain
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119253pJMP1171rfp "test" strain
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119254pJMP1183rfp "test" strain
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119255pJMP1185rfp "test" strain
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119256pJMP1187rfp "test" strain
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119257pJMP1189rfp "test" strain
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119258pJMP1217rfp "test" strain
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119259pJMP1219rfp "test" strain
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119260pJMP1221rfp "test" strain
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119261pJMP1223rfp "test" strain
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119262pJMP1237for cloning new sgRNAs
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119263pJMP1239knockdown folA in P. aeruginosa
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119264pJMP1263B. subtilis attTn7 site and flanking sequence cloned into pACYC
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119265pJMP1273E. coli attTn7 site with B. subtilis flanking sequence cloned into pACYC
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119266pJMP1274B. subtilis deltaTn7 site and flanking sequence cloned into pACYC
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119267pJMP1290construction intermediate
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119268pJMP1333rfp "test" strain
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119269pJMP1335rfp "test" strain
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119270pJMP1337for cloning new sgRNAs
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119271pJMP1339for cloning new sgRNAs
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119272pJMP1341control sgRNA in cells lacking rfp
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119273pJMP1344knockdown folA in E. aerogenes
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119274pJMP1346knockdown folA in K. pneumoniae
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119275pJMP1354for cloning new sgRNAs
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119276pJMP1356for cloning new sgRNAs
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119277pJMP1358for cloning new sgRNAs
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119278pJMP1360for cloning new sgRNAs
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119279pJMP1363for stabilizing ICE in the presence of rapI expression
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