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Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.
Hu JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR
Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
108379xCas9 3.7Mammalian expression vector for xCas9 3.7
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108380xCas9(3.7)-BE3Mammalian expression vector for xCas9(3.7)-BE3
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108381xCas9(3.7)-BE4Mammalian expression vector for xCas9(3.7)-BE4
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108382xCas9(3.7)-ABE(7.10)Mammalian expression vector for xCas9(3.7)-ABE
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108383dxCas(3.7)-VPRMammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus
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108384xCas9 3.6Mammalian expression vector for xCas9 3.6
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108385xCas9(3.6)-BE3Mammalian expression vector for xCas9(3.6)-BE3
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108386xCas9(3.6)-BE4Mammalian expression vector for xCas9(3.6)-BE4
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108387xCas9(3.6)-ABE(7.10)Mammalian expression vector for xCas9(3.6)-ABE
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108388dxCas(3.6)-VPRMammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus
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