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dxCas(3.6)-VPR
(Plasmid #108388)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108388 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dxCas(3.6)-VPR
  • Alt name
    dxCas9(3.6)-NLS-VP64-p65-Rta
  • Species
    Synthetic
  • Insert Size (bp)
    5799
  • Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, E1219V
  • Tag / Fusion Protein
    • NLS (N terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dxCas(3.6)-VPR was a gift from David Liu (Addgene plasmid # 108388)
  • For your References section:

    Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Hu JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. 10.1038/nature26155 PubMed 29512652