164,060 results

1. pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro

   Plasmid

   #129537

   Purpose

   PiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

   Depositor

   Hideyuki Okano

   Article

   Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

   Insert

   tetR-3xFlag-HA-2A-Puro
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   hPGK promoter

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Anti-WAVE1/SCAR [K91/36R]

   Plasmid

   #128629

   Purpose

   Mammalian Expression Plasmid of anti-WAVE1/SCAR (Human). Derived from hybridoma K91/36.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-WAVE1/SCAR (Human) recombinant mouse monoclonal antibody (WASF1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-UbiC-GFP-ps

   Plasmid

   #183712

   Purpose

   GFP with palmitoylation signal

   Depositor

   Jeffrey Gerst

   Article

   Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

   Insert

   GFP with palmitoylation signal
   

   Use

   Lentiviral

   Tags

   palmitoylation signal

   Expression

   Mutation

   Promoter

   UbiC

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBPZpGAL4DBDUw

   Plasmid

   #26233

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   Zip-GAL4DBD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEM270 - MCS mosTI hygroR target

   Plasmid

   #159826

   Purpose

   Single copy or array insertion by MosTI using split hygroR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   MCS mosTI hygroR target
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-Rab7A

   Plasmid

   #131417

   Purpose

   Expresses HA-tagged human Rab7A in mammalian cells

   Depositor

   Ken-Ichi Takemaru, FENG-QIAN Li

   Insert

   human Rab7A
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAW12.lentiguide.GFP

   Plasmid

   #104374

   Purpose

   GFP vector for cloning in guides

   Depositor

   Richard Young

   Article

   Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-OXA1L

   Plasmid

   #116768

   Purpose

   Lentiviral expression of OXA1L

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   OXA1L (OXA1L Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. (401) pAAV CMV-ZsGreen U6-gRNA

   Plasmid

   #163021

   Purpose

   Fluorescent reporter for Sa gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Zs Green
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUltra-U6-gRNAs1-5

    Plasmid

    #178569

    Purpose

    Constitutive expression of a gRNA-tRNA array for macsGESTALT editing

    Depositor

    Christopher Lengner

    Article

    Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

    Insert

    5 gRNA-tRNA array with U6 promoter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xHA-miniTurbo-NLS_pCDNA3

    Plasmid

    #107172

    Purpose

    expresses 3xHA-tagged miniTurbo in the mammalian nucleus

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-HA-MyD88-FW/AA

    Plasmid

    #12289

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    MyD88 (MYD88 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F285A/W286A

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

13. PM-FRB-mRFP-T2A-FKBP-5-ptase

    Plasmid

    #40896

    Depositor

    Peter Varnai

    Article

    Toth et al J Cell Sci. 2012 May 1;125(Pt 9):2185-97

    Insert

    PM-FRB-mRFP-T2A-FKBP-5-ptase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. hNCAM2

    Plasmid

    #84473

    Purpose

    expression of hNCAM2 in mammalian cells

    Depositor

    Vladimir Sytnyk

    Article

    Leshchyns'ka et al Nat Commun. 2015 Nov 27;6:8836. doi: 10.

    Insert

    NCAM2 (NCAM2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-mCardinal

    Plasmid

    #51311

    Purpose

    Expresses far-red fluorescent protein mCardinal in mammalian cells

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mCardinal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD_FLAG-GFP(Y39TAG)-6His

    Plasmid

    #223510

    Purpose

    Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

    Depositor

    Edward Lemke

    Article

    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

    Insert

    GFP(Y39TAG)
    

    Use

    Tags

    6xHis and FLAG

    Expression

    Bacterial

    Mutation

    Y39TAG

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEM321 - array co-injection fragment mosTI hygroR

    Plasmid

    #159828

    Purpose

    Single copy or array insertion by MosTI using split hygroR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI hygroR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #190034

    Purpose

    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized hfCas13d
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A134V,A140V,A141V,A143V

    Promoter

    CBh, SV40, hU6

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSPTg.T2FXK (#54)

    Plasmid

    #35963

    Depositor

    Thomas Sato

    Article

    Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

    Inserts

    Tie2 promoter
    Tie2 enhancer
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pTriEx4-Cdc42-2G

    Plasmid

    #68814

    Purpose

    FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

    Depositor

    Olivier Pertz

    Article

    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

    Insert

    Cdc42 activation reporter
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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