|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20184||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameNotch 2 intracellular domain
Alt nameNotch 2
SpeciesM. musculus (mouse)
Insert Size (bp)2403
MutationContains murine Notch 2 Intracellular Domain (Met-1699 to Ala-2470)
GenBank IDNM_010928.2 NP_035058.2
Entrez GeneNotch2 (a.k.a. AI853703, N2)
- Promoter CMV
/ Fusion Protein
- 3X FLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-pA-R (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byNotch 2 plasmid from Dr. Yoshio Hamada (NIBB, Japan)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD2 was cloned into XbaI/BamHI sites. NICD2 (Met-1699 to Ala-2470 of full-length mouse N2) was amplified from the N2 plasmid (gift from Dr. Y. Hamada) using primers 5XbaN2 (5'-GCTCTAGAATGGCCAAGCGGAAGCGCAAG-3') and 3BamN2
Please note that Addgene sequencing results confirm the updated domain numbering to be Met 1701-Ala 2473.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:3XFlagNICD2 was a gift from Raphael Kopan (Addgene plasmid # 20184 ; http://n2t.net/addgene:20184 ; RRID:Addgene_20184)
For your References section:Target selectivity of vertebrate notch proteins. Collaboration between discrete domains and CSL-binding site architecture determines activation probability. Ong CT, Cheng HT, Chang LW, Ohtsuka T, Kageyama R, Stormo GD, Kopan R. J Biol Chem. 2006 Feb 24. 281(8):5106-19. 10.1074/jbc.M506108200 PubMed 16365048