pCS2 Notch1 Full Length-6MT
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||41728||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4352
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameNotch-1 Full Length
SpeciesM. musculus (mouse)
MutationL1997V; Contains murine Notch-1 protein up to amino acid 2184
GenBank IDAAM28905.1 AF508809_1
Entrez GeneNotch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
/ Fusion Protein
- 6xMyc (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer Sp6
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Alternate plasmid name: pCS2 NFL6MT
pCS+ NFL contains the Notch-1 protein up to amino acid 2184 fused to a hexameric Myc tag at the carboxy terminus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2 Notch1 Full Length-6MT was a gift from Raphael Kopan (Addgene plasmid # 41728 ; http://n2t.net/addgene:41728 ; RRID:Addgene_41728)
For your References section:Notch-1 signalling requires ligand-induced proteolytic release of intracellular domain. Schroeter EH, Kisslinger JA, Kopan R. Nature. 1998 May 28;393(6683):382-6. 10.1038/30756 PubMed 9620803