pCS2 Notch1 ICv-6MT
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||41730||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4352
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNotch-1 Intracellular Domain
Alt nameNotch1 Intracellular Domain
SpeciesM. musculus (mouse)
MutationContains murine Notch-1 protein beginning at Valine 1744 (aa 1744-2184)
GenBank IDNP_032740 BAC77039.1
/ Fusion Protein
- 6xMyc (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer Sp6
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Alternate plasmid name: pCS2 NICv1744-6MT
To create pCS2+ NICV1744, the primer CCAGGATCCACCATGGTGCTGCTGTCCCGCAAGC was used with primer M95 (5'-TCGAACATTGACATCCATGCA-3') to generate a product that was digested with Bam HI and Bcl I, and ligated into the same sites in NΔE (Addgene plasmid #41737).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2 Notch1 ICv-6MT was a gift from Raphael Kopan (Addgene plasmid # 41730 ; http://n2t.net/addgene:41730 ; RRID:Addgene_41730)
For your References section:Notch-1 signalling requires ligand-induced proteolytic release of intracellular domain. Schroeter EH, Kisslinger JA, Kopan R. Nature. 1998 May 28;393(6683):382-6. 10.1038/30756 PubMed 9620803