pCS2 Notch1 ΔEMV-6MT
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||41737||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4352
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNotch-1 Extracellular-domain-deleted construct
Alt nameN1 Extracellular-domain-deleted construct
SpeciesM. musculus (mouse)
MutationContains murine Notch-1 protein fragment lacking the extracellular region [contains murine Notch-1 leader peptide (aa 1-23) and then aa 1704-2184]; M1727V
GenBank IDAAM28905.1 AF508809_1
/ Fusion Protein
- 6xMyc (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer Sp6
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Alternate plasmid name: pCS2 NΔE-6MT
All Notch1 constructs deposited here were cloned into the pCS2+MT vector (Rupp et al., 1994; Turner and Weintraub, 1994) and have the C-terminal 348 residues (aa 2185 to C terminus) of Notch replaced with a hexameric myc tag to facilitate biochemical analysis.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2 Notch1 ΔEMV-6MT was a gift from Raphael Kopan & Jeffrey Nye (Addgene plasmid # 41737 ; http://n2t.net/addgene:41737 ; RRID:Addgene_41737)
For your References section:Signal transduction by activated mNotch: importance of proteolytic processing and its regulation by the extracellular domain. Kopan R, Schroeter EH, Weintraub H, Nye JS. Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1683-8. 10.1073/pnas.93.4.1683 PubMed 8643690
Map uploaded by the depositor.