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pCS2 Notch1 ΔEMV-6MT
(Plasmid #41737)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41737 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Notch-1 Extracellular-domain-deleted construct
  • Alt name
    Notch1
  • Alt name
    N1 Extracellular-domain-deleted construct
  • Alt name
    N1
  • Species
    M. musculus (mouse)
  • Mutation
    Contains murine Notch-1 protein fragment lacking the extracellular region [contains murine Notch-1 leader peptide (aa 1-23) and then aa 1704-2184]; M1727V
  • GenBank ID
    AAM28905.1 AF508809_1
  • Tag / Fusion Protein
    • 6xMyc (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Full length Notch-1 cDNA from Jeffrey S. Nye (Northwestern University School of Medicine, Chicago, IL)
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Alternate plasmid name: pCS2 NΔE-6MT

All Notch1 constructs deposited here were cloned into the pCS2+MT vector (Rupp et al., 1994; Turner and Weintraub, 1994) and have the C-terminal 348 residues (aa 2185 to C terminus) of Notch replaced with a hexameric myc tag to facilitate biochemical analysis.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2 Notch1 ΔEMV-6MT was a gift from Raphael Kopan & Jeffrey Nye (Addgene plasmid # 41737 ; http://n2t.net/addgene:41737 ; RRID:Addgene_41737)
  • For your References section:

    Signal transduction by activated mNotch: importance of proteolytic processing and its regulation by the extracellular domain. Kopan R, Schroeter EH, Weintraub H, Nye JS. Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1683-8. 10.1073/pnas.93.4.1683 PubMed 8643690