|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20183||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameNotch 1 Intracellular Domain
Alt nameNotch 1
SpeciesM. musculus (mouse)
Insert Size (bp)2361
MutationContains murine Notch 1 Intracellular Domain (Val-1744 to Lys-2531)
GenBank IDAAM28905.1 AF508809_1
Entrez GeneNotch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
- Promoter CMV
/ Fusion Protein
- 3XFLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD1 was cloned into XbaI/BamHI sites. NICD1 (Val-1744 to Lys-2531 of full-length mouse N1) was amplified using primers 5XbaN1 (5'-GCCTCTAGAGTGCTGCTGTCCCGCAAGCGC-3') and 3BamN1 (5'-CGGGATCCTTATTTAAATGCCTCTGGAATGTGGG-3').
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:3XFlagNICD1 was a gift from Raphael Kopan (Addgene plasmid # 20183 ; http://n2t.net/addgene:20183 ; RRID:Addgene_20183)
For your References section:Target selectivity of vertebrate notch proteins. Collaboration between discrete domains and CSL-binding site architecture determines activation probability. Ong CT, Cheng HT, Chang LW, Ohtsuka T, Kageyama R, Stormo GD, Kopan R. J Biol Chem. 2006 Feb 24. 281(8):5106-19. 10.1074/jbc.M506108200 PubMed 16365048