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3XFlagNICD1
(Plasmid #20183)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20183 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p3XFLAG-CMV-7
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Notch 1 Intracellular Domain
  • Alt name
    NICD1
  • Alt name
    Notch 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2361
  • Mutation
    Contains murine Notch 1 Intracellular Domain (Val-1744 to Lys-2531)
  • GenBank ID
    AAM28905.1 AF508809_1
  • Entrez Gene
    Notch1 (a.k.a. 9930111A19Rik, Mi, Mis6, N, N1, Ta, Tan1, lin, lin-12)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3XFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Full length Notch-1 cDNA from Jeffrey S. Nye (Northwestern University School of Medicine, Chicago, IL)
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD1 was cloned into XbaI/BamHI sites. NICD1 (Val-1744 to Lys-2531 of full-length mouse N1) was amplified using primers 5XbaN1 (5'-GCCTCTAGAGTGCTGCTGTCCCGCAAGCGC-3') and 3BamN1 (5'-CGGGATCCTTATTTAAATGCCTCTGGAATGTGGG-3').

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3XFlagNICD1 was a gift from Raphael Kopan (Addgene plasmid # 20183 ; http://n2t.net/addgene:20183 ; RRID:Addgene_20183)
  • For your References section:

    Target selectivity of vertebrate notch proteins. Collaboration between discrete domains and CSL-binding site architecture determines activation probability. Ong CT, Cheng HT, Chang LW, Ohtsuka T, Kageyama R, Stormo GD, Kopan R. J Biol Chem. 2006 Feb 24. 281(8):5106-19. 10.1074/jbc.M506108200 PubMed 16365048