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Live imaging of mRNA using RNA-stabilized fluorogenic proteins.
Wu J, Zaccara S, Khuperkar D, Kim H, Tanenbaum ME, Jaffrey SR
Nat Methods. 2019 Sep;16(9):862-865. doi: 10.1038/s41592-019-0531-7. Epub 2019 Aug 30. PubMed Article

Plasmids from Article

ID Plasmid Purpose
129401CMV-mCherry-(F30-2xPepper)10mCherry mRNA reporter tagged with (F30-2xPepper)10
129402miniCMV-(mNeonGreen)4-tDeg(mNeonGreen)4-tDeg fluorogenic protein
129403CMV-CytERM-mCherry-(F30-2xPepper)10CytERM-mCherry mRNA reporter tagged with (F30-2xPepper)10
129404UbC-(mNeonGreen)4-tDeg(mNeonGreen)4-tDeg fluorogenic protein
129405pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)circular Pepper RNA
129406pAV-U6+27-Tornado-F30-TAR Variant-1circular TAR Variant-1

Antibodies from Article