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Kristala Prather Lab Plasmids


The Kristala Prather Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Combining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control. Leonard E, Ajikumar PK, Thayer K, Xiao WH, Mo JD, Tidor B, Stephanopoulos G, Prather KL Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13654-9. Epub 2010 Jul 19.
Biosynthesis of chiral 3-hydroxyvalerate from single propionate-unrelated carbon sources in metabolically engineered E. coli. Tseng HC, Harwell CL, Martin CH, Prather KL Microb Cell Fact. 2010 Nov 27;9:96.
Synthesis and accumulation of aromatic aldehydes in an engineered strain of Escherichia coli. Kunjapur AM, Tarasova Y, Prather KL J Am Chem Soc. 2014 Aug 20;136(33):11644-54. doi: 10.1021/ja506664a. Epub 2014 Aug 8.
The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli. Reisch CR, Prather KL Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
37853MG1655 ΔendA ΔrecAE. coli bacterial strainCombining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control. Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13654-9. Epub 2010 Jul 19. N/A Add to Cart
37854MG1655 ΔendA ΔrecA (DE3)E. coli bacterial strainBiosynthesis of chiral 3-hydroxyvalerate from single propionate-unrelated carbon sources in metabolically engineered E. coli. Microb Cell Fact. 2010 Nov 27;9:96. N/A Add to Cart
61440E. coli K-12 MG1655 RARESynthesis and accumulation of aromatic aldehydes in an engineered strain of Escherichia coli. J Am Chem Soc. 2014 Aug 20;136(33):11644-54. doi: 10.1021/ja506664a. Epub 2014 Aug 8. This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes E. coli K-12 MG1655 genome Add to Cart
62654pKDsgRNA-ackexo, beta, gam, sgRNABacterial ExpressionThe no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli. Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096. Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression pKD46 Add to Cart
62655pCas9-CR4Cas9 nucleaseBacterial ExpressionThe no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli. Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096. Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR pdCas9-bacteria Add to Cart
62656pKDsgRNA-p15exo, beta, gam, sgRNABacterial ExpressionThe no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli. Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096. Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid pKD46 Add to Cart