pLacO-ISce1
(Plasmid #58505)

• Purpose: provides 256 Lac operator repeats adjacent to the ISce1 restriction site
• Depositing Lab: Tom Misteli
• Publication: Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi: 10.1126/science.1237150.

Backbone

• Vector backbone: pBluescript II KS
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 13500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Growth instructions: Plasmids are transformed in Stbl2 competent cells (Invitrogen). Grow always in 30oC to avoid recombination events and check the digestion pattern (e.g. cut with XhoI-SacII). Check this every time you propagate these plasmids, since recombination events are very often.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Lac repeats adjacent to 6 copies of the ISce1 restriction site
• Species: bacterial lac operator repeats
• Insert Size (bp): 10000
• Promoter: no promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: M13 pUC forward primer
• 3′ sequencing primer: M13pUC reverse primer

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NOTE: Though EcoRI and XbaI are shown as being in the MCS on the depositor's map, they are NOT unique in this plasmid. We do not recommend them for cloning.