AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE
(Plasmid #51509)

• Purpose: Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter
• Depositing Lab: Hongkui Zeng
• Publication: Oh et al Nature. 2014 Apr 10;508(7495):207-14. doi: 10.1038/nature13186. Epub 2014 Apr 2.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	51509	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pAAV-MCS
  (Search Vector Database)
• Backbone manufacturer: Stratagene
• Total vector size (bp): 7634
• Modifications to backbone: A fragment containing the phSyn promoter-FLEX-tdTomato-T2A-synaptophysin fused EGFP-WPRE-bGHpA was swapped in to replace the CMV promoter-MCS-hGHpA sequence. Total insert size including the two ITRs is 5037 bp.
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tdTomato-T2A-SypEGFP
• Alt name: tdTomato
• Alt name: EGFP
• Alt name: Synaptophysin
• Insert Size (bp): 1428
• Promoter: phSyn1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• Terms and Licenses:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
  • Ancillary Agreement for Plasmids Containing FP Materials
  • genOway Notice of RIghts
• Articles Citing this Plasmid:
  • 3 References