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CAG Double flox Synaptophysin-EGFP WPRE
(Plasmid #73816)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73816 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV CAG
  • Backbone size w/o insert (bp) 6137
  • Total vector size (bp) 7823
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synaptophysin-eGFP
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1686
  • GenBank ID
    NM_012664
  • Promoter CAG
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site Asc1 (not destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The gene synaptophysin-eGFP (77504-1) was cloned in D13 AAV CAG Double ox eGFP by EcoRV and Asc1. This construct can be electroporated into the uteros or packed into a virus for viral injection

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG Double flox Synaptophysin-EGFP WPRE was a gift from Michael Stryker (Addgene plasmid # 73816 ; http://n2t.net/addgene:73816 ; RRID:Addgene_73816)
  • For your References section:

    Sonic hedgehog expression in corticofugal projection neurons directs cortical microcircuit formation. Harwell CC, Parker PR, Gee SM, Okada A, McConnell SK, Kreitzer AC, Kriegstein AR. Neuron. 2012 Mar 22;73(6):1116-26. doi: 10.1016/j.neuron.2012.02.009. Epub 2012 Mar 21. 10.1016/j.neuron.2012.02.009 PubMed 22445340