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Parkin mediates nonclassical, proteasomal-independent ubiquitination of synphilin-1: implications for Lewy body formation.
Lim KL, Chew KC, Tan JM, Wang C, Chung KK, Zhang Y, Tanaka Y, Smith W, Engelender S, Ross CA, Dawson VL, Dawson TM
J Neurosci. 2005 Feb 23. 25(8):2002-9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
17602pRK5-HA-Ubiquitin-K29RMammalian expression of HA tagged ubiquitin K29R
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17603pRK5-HA-Ubiquitin-KOMammalian expression of HA tagged ubiquitin carrying zero lysines (K0)
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17604pRK5-HA-Ubiquitin-K48RMammalian expression of HA tagged ubiquitin K48R
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17605pRK5-HA-Ubiquitin-K48Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines
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17606pRK5-HA-Ubiquitin-K63Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines
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17607pRK5-HA-Ubiquitin-K33Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines
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17608pRK5-HA-Ubiquitin-WTMammalian expression of HA tagged ubiquitin
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