|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17603||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameUbiquitin C
SpeciesH. sapiens (human)
Insert Size (bp)220
MutationKO: all lysines mutated to arginines
Entrez GeneUBC (a.k.a. HMG20)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
The ubiquitin KO sequence in this plasmid contains the following lysine to arginine mutations: K6R, K11R, K27R, K29R, K33R, K48R and K63R.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRK5-HA-Ubiquitin-KO was a gift from Ted Dawson (Addgene plasmid # 17603 ; http://n2t.net/addgene:17603 ; RRID:Addgene_17603)
For your References section:Parkin mediates nonclassical, proteasomal-independent ubiquitination of synphilin-1: implications for Lewy body formation. Lim KL, Chew KC, Tan JM, Wang C, Chung KK, Zhang Y, Tanaka Y, Smith W, Engelender S, Ross CA, Dawson VL, Dawson TM. J Neurosci. 2005 Feb 23. 25(8):2002-9. 10.1523/JNEUROSCI.4474-04.2005 PubMed 15728840