|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17607||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameUbiquitin C
SpeciesH. sapiens (human)
Insert Size (bp)220
MutationK33 only, other lysines mutated to arginines; P19S and E24D
Entrez GeneUBC (a.k.a. HMG20)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Please note that Addgene's sequencing results identified P19S and E24D mutations in the ubiquitin sequence. These mutations were not intentional, but were present in the original source clone from which this plasmid was created (see associated publication for details).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRK5-HA-Ubiquitin-K33 was a gift from Ted Dawson (Addgene plasmid # 17607 ; http://n2t.net/addgene:17607 ; RRID:Addgene_17607)
For your References section:Parkin mediates nonclassical, proteasomal-independent ubiquitination of synphilin-1: implications for Lewy body formation. Lim KL, Chew KC, Tan JM, Wang C, Chung KK, Zhang Y, Tanaka Y, Smith W, Engelender S, Ross CA, Dawson VL, Dawson TM. J Neurosci. 2005 Feb 23. 25(8):2002-9. 10.1523/JNEUROSCI.4474-04.2005 PubMed 15728840