pBMN YFP-DHFR(DD)
(Plasmid #29326)

• Depositing Lab: Thomas Wandless
• Publication: Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Backbone

• Vector backbone: pBMN
• Backbone manufacturer: Garry Nolan
• Backbone size w/o insert (bp): 6943
• Vector type: Retroviral
• Selectable markers: Hc-Red-t fluorecent protein behind IRES

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Top10
• Growth instructions: TOP10 cells. Culture at 30C to prevent recombination of LTR and lost insert. Takes >20 hours to get good culture at 30C.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: YFP-DHFR(DD)
• Alt name: folA dihydrofolate reductase ( destable domain) with YFP fusion
• Species: E. coli
• Insert Size (bp): 1233
• Mutation: Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Val 19 Ala (V19A) and Gly 67 to Ser (G67S)--destabilizing domain
• GenBank ID: AAC73159
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • YFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: pBMN 5'

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry