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MSCV-hCD19
(Plasmid #127889)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127889 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 5097
  • Total vector size (bp) 6102
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hCD19
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1005
  • Mutation
    none
  • Entrez Gene
    CD19 (a.k.a. B4, CVID3)
  • Tag / Fusion Protein
    • no tags

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Got gene from NCBI
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Optimal packaging results are obtained using the pCL-10A1 Retrovirus Packaging Vector from Novus Biologicals.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-hCD19 was a gift from Anjana Rao (Addgene plasmid # 127889 ; http://n2t.net/addgene:127889 ; RRID:Addgene_127889)
  • For your References section:

    NR4A transcription factors limit CAR T cell function in solid tumours. Chen J, Lopez-Moyado IF, Seo H, Lio CJ, Hempleman LJ, Sekiya T, Yoshimura A, Scott-Browne JP, Rao A. Nature. 2019 Mar;567(7749):530-534. doi: 10.1038/s41586-019-0985-x. Epub 2019 Feb 27. 10.1038/s41586-019-0985-x PubMed 30814732