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Addgene

pSLCAR-CD19-BBz
(Plasmid #135992)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135992 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSL
  • Backbone manufacturer
    Heavily modified from LentiCRISPR v1 backbone
  • Modifications to backbone
    Use BpiI single pot restriction ligation to swap the antigen binding domain of the CAR from CD19 to desired specificity
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FMC63-41BB-3z-P2A-EGFP
  • Alt name
    CD19(FMC63)-CAR, 41BB costim., CD3z stim domains, P2A-GFP marker
  • Alt name
    Contains: FMC63-scFV, Hinge domain, CD28-transmembrane domain, 41BB-ITD, CD3z-ITD
  • Species
    H. sapiens (human)
  • Entrez Gene
    CD247 (a.k.a. CD3-ZETA, CD3H, CD3Q, CD3Z, CD3ZETA, IMD25, T3Z, TCRZ)
  • Entrez Gene
    CD28 (a.k.a. Tp44)
  • Entrez Gene
    TNFRSF9 (a.k.a. 4-1BB, CD137, CDw137, ILA, IMD109)
  • Promoter EF1a-short

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BpiI (destroyed during cloning)
  • 3′ cloning site BpiI (destroyed during cloning)
  • 5′ sequencing primer CGGGTTTGCCGCCA
  • 3′ sequencing primer caggagccTGCTCCTCTTACTcc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLCAR-CD19-BBz was a gift from Scott McComb (Addgene plasmid # 135992 ; http://n2t.net/addgene:135992 ; RRID:Addgene_135992)
  • For your References section:

    A High-Throughput Method for Characterizing Novel Chimeric Antigen Receptors in Jurkat Cells. Bloemberg D, Nguyen T, MacLean S, Zafer A, Gadoury C, Gurnani K, Chattopadhyay A, Ash J, Lippens J, Harcus D, Page M, Fortin A, Pon RA, Gilbert R, Marcil A, Weeratna RD, McComb S. Mol Ther Methods Clin Dev. 2020 Jan 31;16:238-254. doi: 10.1016/j.omtm.2020.01.012. eCollection 2020 Mar 13. 10.1016/j.omtm.2020.01.012 PubMed 32083149