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MSCV-myc-CAR-2A-Thy1.1
(Plasmid #127890)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127890 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 5172
  • Total vector size (bp) 7134
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    myc,
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    30

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer 5' MSCV
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    CAR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1386

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer 5' MSCB
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    2A
  • Species
    porcine
  • Insert Size (bp)
    57

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer 5' MSCV
  • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    Thy1.1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    489

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer 5' MSCV
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Pieced together from Cral June and Steve Rosenberg's papers
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-myc-CAR-2A-Thy1.1 was a gift from Anjana Rao (Addgene plasmid # 127890 ; http://n2t.net/addgene:127890 ; RRID:Addgene_127890)
  • For your References section:

    NR4A transcription factors limit CAR T cell function in solid tumours. Chen J, Lopez-Moyado IF, Seo H, Lio CJ, Hempleman LJ, Sekiya T, Yoshimura A, Scott-Browne JP, Rao A. Nature. 2019 Mar;567(7749):530-534. doi: 10.1038/s41586-019-0985-x. Epub 2019 Feb 27. 10.1038/s41586-019-0985-x PubMed 30814732