168,579 results

1. GW1-pHRed

   Plasmid

   #31473

   Purpose

   genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

   Depositor

   Gary Yellen

   Article

   Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

   Insert

   pHRed
   

   Expression

   Mammalian

   Available Since

   July 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATeam1.03-nD/nA/pcDNA3

   Plasmid

   #51958

   Purpose

   mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

   Depositor

   Takeharu Nagai

   Article

   Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

   Insert

   ATeam1.03-nD/nA
   

   Expression

   Mammalian

   Available Since

   March 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pet30b-7M SrtA

   Plasmid

   #51141

   Purpose

   expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

   Depositor

   Hidde Ploegh

   Insert

   S.aureus SrtA 7M
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, E105K, E108Q, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-SIRT1 H363Y

   Plasmid

   #1792

   Depositor

   Michael Greenberg

   Article

   Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

   Insert

   SIRT1 (SIRT1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H363Y, deacetylase domain mutation

   Available Since

   May 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAC150-RFP-EGFP-LGALS3

   Plasmid

   #175778

   Purpose

   PiggyBac Vector expressing GFP-RFP-LGALS3

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   LGALS3 (LGALS3 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. BPK2660

   Plasmid

   #70709

   Purpose

   Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050

   Pooled Library

   #1000000131

   Purpose

   Genome-wide CRISPR gRNA pooled library for inhibition of mouse genes

   Depositor

   John Doench, David Root

   Available Since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pEGFP-N1-IL6

   Plasmid

   #111933

   Purpose

   Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

   Insert

   Interleukin 6 (IL6 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells

   Promoter

   CMV

   Available Since

   July 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-DIO-EYFP

   Plasmid

   #104052

   Purpose

   An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Has Service

   AAV PHP.V1

   Insert

   EYFP
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCXB-EBNA1

    Plasmid

    #41857

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    EBNA-1 (EBNA-1 human herpesvirus 4)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMito-TrxRFP1

    Plasmid

    #98997

    Purpose

    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized

    Depositor

    Huiwang Ai

    Article

    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

    Insert

    TrxRFP1 and mitochondrial localization sequence
    

    Tags

    His-tag and Mitochondria localization sequence

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-FLEX-TeLC-P2A-dTomato

    Plasmid

    #159102

    Purpose

    Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

    Depositor

    Sandeep Datta

    Article

    Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

    Insert

    TeLC-P2A-NLS-dTomato
    

    Use

    AAV

    Promoter

    hSynapsin

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAWP78-PVCpnf_Δpvc10

    Plasmid

    #198448

    Purpose

    Produces PVCs containing truncated spike tip protein (Pvc10).

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_Δpvc10
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM5505

    Plasmid

    #132664

    Purpose

    pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Insert

    MobA
    

    Expression

    Bacterial

    Mutation

    please see depositor comments below

    Promoter

    p1,p3

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-Cre-P2A-dTomato (AAV2)

    Viral Prep

    #107738-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Rylan Larsen

    Promoter

    Syn

    Tags

    dTomato (physically separate, not a fusion protein)

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

16. pAAV_BiPVe4_dTomato_nlsdTomato

    Plasmid

    #213936

    Purpose

    AAV construct expressing dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_dTomato_nlsdTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB and AAV1

    Insert

    dTomato_P2A_nls.dTomato
    

    Use

    AAV

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p6352 MSCV-CMV-CMV-Flag-HA-JMJD6

    Plasmid

    #31358

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    JMJD6 (JMJD6 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    Oct. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-DEST53 PINK1 N-GFP

    Plasmid

    #13315

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

    Plasmid

    #83841

    Purpose

    Fluorescent probe for G1/S transition and M/G1 transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Human, Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-MmPeg10rc4

    Plasmid

    #174858

    Purpose

    Expresses recoded mouse Peg10 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    recoded Mus musculus PEG10 (Peg10 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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