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Visualization of actin filaments and monomers in somatic cell nuclei.
Belin BJ, Cimini BA, Blackburn EH, Mullins RD
Mol Biol Cell. 2013 Apr;24(7):982-94. doi: 10.1091/mbc.E12-09-0685. Epub 2013 Feb 27.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
58466pEGFP-C1 Utr230-EGFP-3XNLSExpresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter
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58467pEGFP-C1 Lifeact-EGFP-2XNLSExpresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter
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58468pEGFP-C1 EGFP-3XNLSExpresses a nuclear-targeted eGFP on a CMV promoter
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58469pEGFP-C1 RPEL1-EGFP-3XNLSExpresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter
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Antibodies from Article