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pEGFP-C1 RPEL1-EGFP-3XNLS
(Plasmid #58469)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58469 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4686
  • Total vector size (bp) 4797
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    MRTF-A RPEL1
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
    99
  • GenBank ID
    AF532597
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer EGFP Reverse
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    3X NLS with 3X stop codons
  • Species
    SV40 virus
  • Insert Size (bp)
    72
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer EGFP Forward
  • 3′ sequencing primer SV40 polyA Reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1 RPEL1-EGFP-3XNLS was a gift from Dyche Mullins (Addgene plasmid # 58469)
  • For your References section:

    Visualization of actin filaments and monomers in somatic cell nuclei. Belin BJ, Cimini BA, Blackburn EH, Mullins RD. Mol Biol Cell. 2013 Apr;24(7):982-94. doi: 10.1091/mbc.E12-09-0685. Epub 2013 Feb 27. 10.1091/mbc.E12-09-0685 PubMed 23447706