Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Cas9 gRNA engineering for genome editing, activation and repression.
Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR, Collins JJ, Weiss R, Church G
Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
68495AAV_NLS-dSaCas9-NLS-VPRAAV vector containing nuclease null SaCas9 fused to VPR
Loading...
68496AAV_NLS-SaCas9-NLS-VPRAAV vector containing SaCas9 fused to VPR
Loading...
68497hCas9-VPRnuclease competent SP-Cas9 fused to VPR
Loading...
68498M-ST1-VPRnuclease competent ST1-Cas9 fused to VPR
Loading...
79368gRNA_tdtomato_reporter_1_ST1_2x_bindingAssays activity of transcriptional activators fused to ST1 Cas9
Loading...
79369gRNA_tdtomato_reporter_1_SA-2xAssays activity of transcriptional activators fused to SA Cas9
Loading...
79370gRNA_tdtomato_reporter1_SP-2xAssays activity of transcriptional activators fused to SP Cas9
Loading...